Review




Structured Review

Proteintech 1 ap
1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1001 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/14695+1+ap/Collagen+Type+I+Polyclonal+antibody/pm42785307-190-25-24
Average 96 stars, based on 1001 article reviews
1 ap - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

other:

Article Title: A TGF-β1/LEF1/β-catenin/JLP network motif regulates autophagy and tubule injury in renal fibrosis.
Article Snippet: siRNAs 537 (si-Ctrl and si-LEF1) were obtained from Sangon Biotech.

Article Title: Decoding HuH-7: a comprehensive genetic and molecular portrait of a widely used hepatocellular carcinoma model
Article Snippet: Collagen I , 14695-1-AP , AB_2082037 , Proteintech , 1:1,000 , r pAb.

Incubation:

Article Title: iPSC-MSC-derived exosomes attenuate pulmonary fibrosis by inhibiting pulmonary fibroblast activation via delivery of TRIM31.
Article Snippet: Idiopathic pulmonary fibrosis (IPF) is a serious and progressive lung disease characterized by devastating and progressive fibrosis.. Treatment is unsatisfactory.. There is accumulating evidence that transplantation of mesenchymal stem cell derived exosomes (MSC-EXOs) protects against IPF.

Article Title: (Pro)renin receptor promotes cardiomyocyte senescence via tripartite motif-containing 24-mediated stabilization of p53 in diabetic cardiomyopathy.
Article Snippet: .. After blocking endogenous peroxidase and nonspecific binding, the sections were incubated overnight at 4°C with primary antibodies diluted in 1% BSA: anti-p16 (sc-1661, 1:200; Santa Cruz, Dallas, USA), anti-collagen I (COL I, 14695-1-AP, 1:1000; Proteintech, Wuhan, China), anti-p21 (sc-6246, 1:200; Santa Cruz), anti-PRR Wang et al. Acta Biochim Biophys Sin 2026 (10926-1-AP, 1:400; Proteintech), and anti-collagen III (COL III; 22734-1-AP, 1:1000; Proteintech) antibodies. ..

Article Title: A Biocompatible CMCHMA/F127DA Hydrogel with ROS-Scavenging Activity Prevents Postoperative Peritoneal Adhesions
Article Snippet: Proteins were then transferred onto polyvinylidene fluoride (PVDF) membranes (sourced from Millipore, Bedford, MA, USA). .. The PVDF membranes were blocked in 5% bovine serum albumin (BSA) at room temperature for 1 h.Next,they were incubated with primary antibodies, including rabbit anti-Collagen I (14695-1-AP, 1:1000, Proteintech), rabbit anti-α-SMA (A2235, 1:1000, Abclonal), rabbit anti-P-P65 (82335-1-RR, 1:2000, Proteintech), rabbit anti-P65 (10745-1-AP, 1:1000, Proteintech), rabbit anti-IkBα (ab97783, 1:1000, Abcam), rabbit anti-P-IkBα (ab24783, 1:1000, Abcam) and mouse anti-GAPDH (60004-1-Ig, 1:50,000, Proteintech) at 4 °C overnight. ..

Inhibition:

Article Title: STC-1 improves scar remodeling and is associated with PI3K/AKT signaling and immune modulation.
Article Snippet: Background: Dysregulation of the wound remodeling phase can lead to excessive scar formation, which may cause functional impairment and aesthetic distress.. However, effective strategies to improve tissue repair outcomes remain limited.. Stanniocalcin-1 (STC-1), a secreted glycoprotein, has recently attracted attention for its roles in tissue repair and inflammation, yet its involvement in skin scar remodeling remains unknown.

Biomarker Discovery:

Article Title: STC-1 improves scar remodeling and is associated with PI3K/AKT signaling and immune modulation.
Article Snippet: Background: Dysregulation of the wound remodeling phase can lead to excessive scar formation, which may cause functional impairment and aesthetic distress.. However, effective strategies to improve tissue repair outcomes remain limited.. Stanniocalcin-1 (STC-1), a secreted glycoprotein, has recently attracted attention for its roles in tissue repair and inflammation, yet its involvement in skin scar remodeling remains unknown.

Blocking Assay:

Article Title: (Pro)renin receptor promotes cardiomyocyte senescence via tripartite motif-containing 24-mediated stabilization of p53 in diabetic cardiomyopathy.
Article Snippet: .. After blocking endogenous peroxidase and nonspecific binding, the sections were incubated overnight at 4°C with primary antibodies diluted in 1% BSA: anti-p16 (sc-1661, 1:200; Santa Cruz, Dallas, USA), anti-collagen I (COL I, 14695-1-AP, 1:1000; Proteintech, Wuhan, China), anti-p21 (sc-6246, 1:200; Santa Cruz), anti-PRR Wang et al. Acta Biochim Biophys Sin 2026 (10926-1-AP, 1:400; Proteintech), and anti-collagen III (COL III; 22734-1-AP, 1:1000; Proteintech) antibodies. ..

Binding Assay:

Article Title: (Pro)renin receptor promotes cardiomyocyte senescence via tripartite motif-containing 24-mediated stabilization of p53 in diabetic cardiomyopathy.
Article Snippet: .. After blocking endogenous peroxidase and nonspecific binding, the sections were incubated overnight at 4°C with primary antibodies diluted in 1% BSA: anti-p16 (sc-1661, 1:200; Santa Cruz, Dallas, USA), anti-collagen I (COL I, 14695-1-AP, 1:1000; Proteintech, Wuhan, China), anti-p21 (sc-6246, 1:200; Santa Cruz), anti-PRR Wang et al. Acta Biochim Biophys Sin 2026 (10926-1-AP, 1:400; Proteintech), and anti-collagen III (COL III; 22734-1-AP, 1:1000; Proteintech) antibodies. ..

Western Blot:

Article Title: STING deficiency alleviates scar formation after glaucoma filtration surgery by suppressing p38 MAPK-induced inflammation in mice
Article Snippet: Ang II was purchased from Sigma-Aldrich (A9252, St. Louis, MO, USA). .. The following antibodies were used for Western blotting: anti-phospho-p38 (28796-1-AP, ProteinTech, China), anti-p38 (14064-1-AP, ProteinTech, China), anti-α-smooth muscle actin (α-SMA) (14395-1-AP, ProteinTech, China), anti-collagen I (14695-1-AP, ProteinTech, China), and anti-STING (13647, Cell Signaling Technology, USA). .. The following antibodies were used for immunofluorescence and immunohistochemistry: anti-STING (13647, Cell Signaling Technology, USA), anti-α-SMA (14395-1-AP, ProteinTech, China), anti-phospho-tank-binding kinase 1 (TBK1) (5483, Cell Signaling Technology, USA), anti-phospho-interferon regulatory factor 3 (IRF3) (29047, Cell Signaling Technology, USA), anti-phospho-p65 (3033, Cell Signaling Technology, USA), and anti-F4/80 (R013726, Epizyme, China).



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MI induces atrial fibrosis and upregulates the EZH2/H3K27me3 signaling in mice . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the sham and MI groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the Sham and MI groups ( n = 6). (F) qPCR analysis of the expression levels of Ezh2 mRNA in the atria of mice from the Sham and MI groups ( n = 6). (G,H) Western blot analysis and quantitative analysis of the expression levels of EZH2 ( n = 6) and H3K27me3 ( n = 9) in the atria of mice from the Sham and MI groups. Sham, Sham operation group; MI, myocardial infarction group; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1; EZH2, Enhancer of Zeste Homolog 2; H3K27me3, trimethylated Histone H3 at Lysine 27. Values are displayed as mean ± standard error of the mean.
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MI induces atrial fibrosis and upregulates the EZH2/H3K27me3 signaling in mice . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the sham and MI groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the Sham and MI groups ( n = 6). (F) qPCR analysis of the expression levels of Ezh2 mRNA in the atria of mice from the Sham and MI groups ( n = 6). (G,H) Western blot analysis and quantitative analysis of the expression levels of EZH2 ( n = 6) and H3K27me3 ( n = 9) in the atria of mice from the Sham and MI groups. Sham, Sham operation group; MI, myocardial infarction group; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1; EZH2, Enhancer of Zeste Homolog 2; H3K27me3, trimethylated Histone H3 at Lysine 27. Values are displayed as mean ± standard error of the mean.
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MI induces atrial fibrosis and upregulates the EZH2/H3K27me3 signaling in mice . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the sham and MI groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the Sham and MI groups ( n = 6). (F) qPCR analysis of the expression levels of Ezh2 mRNA in the atria of mice from the Sham and MI groups ( n = 6). (G,H) Western blot analysis and quantitative analysis of the expression levels of EZH2 ( n = 6) and H3K27me3 ( n = 9) in the atria of mice from the Sham and MI groups. Sham, Sham operation group; MI, myocardial infarction group; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1; EZH2, Enhancer of Zeste Homolog 2; H3K27me3, trimethylated Histone H3 at Lysine 27. Values are displayed as mean ± standard error of the mean.
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MI induces atrial fibrosis and upregulates the EZH2/H3K27me3 signaling in mice . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the sham and MI groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the Sham and MI groups ( n = 6). (F) qPCR analysis of the expression levels of Ezh2 mRNA in the atria of mice from the Sham and MI groups ( n = 6). (G,H) Western blot analysis and quantitative analysis of the expression levels of EZH2 ( n = 6) and H3K27me3 ( n = 9) in the atria of mice from the Sham and MI groups. Sham, Sham operation group; MI, myocardial infarction group; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1; EZH2, Enhancer of Zeste Homolog 2; H3K27me3, trimethylated Histone H3 at Lysine 27. Values are displayed as mean ± standard error of the mean.
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In vitro evaluation of osteogenic differentiation capacity . (A–B) Representative photographs of ALP staining on day 14. (C) Quantitative analysis of ALP activity of ADSCs on day 14. (D–E) Representative photographs of ARS staining on day 21. (F–H) RT-qPCR results for the mRNA expression of Collagen-I, <t>Runx2,</t> OPN. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.
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In vitro evaluation of osteogenic differentiation capacity . (A–B) Representative photographs of ALP staining on day 14. (C) Quantitative analysis of ALP activity of ADSCs on day 14. (D–E) Representative photographs of ARS staining on day 21. (F–H) RT-qPCR results for the mRNA expression of Collagen-I, <t>Runx2,</t> OPN. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.
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In vitro evaluation of osteogenic differentiation capacity . (A–B) Representative photographs of ALP staining on day 14. (C) Quantitative analysis of ALP activity of ADSCs on day 14. (D–E) Representative photographs of ARS staining on day 21. (F–H) RT-qPCR results for the mRNA expression of Collagen-I, <t>Runx2,</t> OPN. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.
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Image Search Results


MI induces atrial fibrosis and upregulates the EZH2/H3K27me3 signaling in mice . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the sham and MI groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the Sham and MI groups ( n = 6). (F) qPCR analysis of the expression levels of Ezh2 mRNA in the atria of mice from the Sham and MI groups ( n = 6). (G,H) Western blot analysis and quantitative analysis of the expression levels of EZH2 ( n = 6) and H3K27me3 ( n = 9) in the atria of mice from the Sham and MI groups. Sham, Sham operation group; MI, myocardial infarction group; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1; EZH2, Enhancer of Zeste Homolog 2; H3K27me3, trimethylated Histone H3 at Lysine 27. Values are displayed as mean ± standard error of the mean.

Journal: Reviews in Cardiovascular Medicine

Article Title: Enhancer of Zeste Homolog 2 Inhibition Attenuates Atrial Fibrillation Post-Myocardial Infarction Through Reduced Atrial Fibrosis

doi: 10.31083/RCM51343

Figure Lengend Snippet: MI induces atrial fibrosis and upregulates the EZH2/H3K27me3 signaling in mice . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the sham and MI groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the Sham and MI groups ( n = 6). (F) qPCR analysis of the expression levels of Ezh2 mRNA in the atria of mice from the Sham and MI groups ( n = 6). (G,H) Western blot analysis and quantitative analysis of the expression levels of EZH2 ( n = 6) and H3K27me3 ( n = 9) in the atria of mice from the Sham and MI groups. Sham, Sham operation group; MI, myocardial infarction group; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1; EZH2, Enhancer of Zeste Homolog 2; H3K27me3, trimethylated Histone H3 at Lysine 27. Values are displayed as mean ± standard error of the mean.

Article Snippet: The antibodies utilized in this study comprised the following: Enhancer of Zeste Homolog 2 (EZH2, 5246S, CST, USA), trimethylated Histone H3 at Lysine 27 (H3K27me3, 9733S, CST, USA), Histone 3 (H3, 9715S, CST, USA), collagen I (14695-1-AP, Proteintech, USA), Transforming Growth Factor-beta1 (TGF-β1, 81746-2-RR, Proteintech, USA), Alpha-Smooth Muscle Actin (α-SMA, 14395-1-AP, Proteintech, USA), and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH, 60004-1-lg, Proteintech, USA).

Techniques: Staining, Analysis, Western Blot, Expressing

Inhibition of EZH2 attenuates atrial fibrosis post-MI . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the MI + Vehicle and MI + GSK126 groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the MI + Vehicle and MI + GSK126 groups ( n = 6). MI + Vehicle, myocardial infarction with vehicle control group; MI + GSK126, myocardial infarction group treated with GSK126; EZH2, Enhancer of Zeste Homolog 2; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1. Values are displayed as mean ± standard error of the mean.

Journal: Reviews in Cardiovascular Medicine

Article Title: Enhancer of Zeste Homolog 2 Inhibition Attenuates Atrial Fibrillation Post-Myocardial Infarction Through Reduced Atrial Fibrosis

doi: 10.31083/RCM51343

Figure Lengend Snippet: Inhibition of EZH2 attenuates atrial fibrosis post-MI . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the MI + Vehicle and MI + GSK126 groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the MI + Vehicle and MI + GSK126 groups ( n = 6). MI + Vehicle, myocardial infarction with vehicle control group; MI + GSK126, myocardial infarction group treated with GSK126; EZH2, Enhancer of Zeste Homolog 2; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1. Values are displayed as mean ± standard error of the mean.

Article Snippet: The antibodies utilized in this study comprised the following: Enhancer of Zeste Homolog 2 (EZH2, 5246S, CST, USA), trimethylated Histone H3 at Lysine 27 (H3K27me3, 9733S, CST, USA), Histone 3 (H3, 9715S, CST, USA), collagen I (14695-1-AP, Proteintech, USA), Transforming Growth Factor-beta1 (TGF-β1, 81746-2-RR, Proteintech, USA), Alpha-Smooth Muscle Actin (α-SMA, 14395-1-AP, Proteintech, USA), and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH, 60004-1-lg, Proteintech, USA).

Techniques: Inhibition, Staining, Analysis, Western Blot, Expressing, Control

In vitro evaluation of osteogenic differentiation capacity . (A–B) Representative photographs of ALP staining on day 14. (C) Quantitative analysis of ALP activity of ADSCs on day 14. (D–E) Representative photographs of ARS staining on day 21. (F–H) RT-qPCR results for the mRNA expression of Collagen-I, Runx2, OPN. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Journal: Materials Today Bio

Article Title: Ultrasound-activated piezoelectric Silk-PVDF hydrogel reprograms the osteoimmune microenvironment via NRF2 signaling for accelerated bone regeneration

doi: 10.1016/j.mtbio.2026.102779

Figure Lengend Snippet: In vitro evaluation of osteogenic differentiation capacity . (A–B) Representative photographs of ALP staining on day 14. (C) Quantitative analysis of ALP activity of ADSCs on day 14. (D–E) Representative photographs of ARS staining on day 21. (F–H) RT-qPCR results for the mRNA expression of Collagen-I, Runx2, OPN. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: Membranes were blocked with 5 % non-fat milk (BD DifcoTM, #232100) in TBST (Beyotime, #ST673) for 1 h at 25 °C and incubated overnight at 4 °C with the following primary antibodies: Osteopontin (OPN) : • Rabbit monoclonal (Proteintech, 22952-1-AP), 1:1000 • Collagen I (COL1A1): Rabbit polyclonal (Proteintech, 14695-1-AP), 1:1000 • RUNX2: Rabbit monoclonal (Proteintech, 20700-1-AP), 1:1000 • NRF2: Rabbit monoclonal (Proteintech, 16396-1-AP), 1:2000 • NQO1: Mouse monoclonal (Proteintech, 67240-1-Ig), 1:2000 • Beta Actin: Mouse monoclonal (Proteintech, 66009-1-Ig), 1:20,000 After three 10-min TBST washes, membranes were incubated with HRP-conjugated goat anti-rabbit/mouse IgG (Beyotime, #A0208/#A0216, 1:5000) for 2 h at 25 °C.

Techniques: In Vitro, Staining, Activity Assay, Quantitative RT-PCR, Expressing

In vitro evaluation of osteogenesis related proteins and genes. (A) The relative protein expression levels of C1, Runx2 and OPN. (B–D) Semi-quantitative analysis of immunoblotting results of C1, Runx2 and OPN. (E) Immunofluorescence staining of OPN. (F) Immunofluorescence staining of OCN. (G) The relative protein expression levels of C1, Runx2 and OPN. (H–J) Semi-quantitative analysis of immunoblotting results of C1, Runx2 and OPN. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Journal: Materials Today Bio

Article Title: Ultrasound-activated piezoelectric Silk-PVDF hydrogel reprograms the osteoimmune microenvironment via NRF2 signaling for accelerated bone regeneration

doi: 10.1016/j.mtbio.2026.102779

Figure Lengend Snippet: In vitro evaluation of osteogenesis related proteins and genes. (A) The relative protein expression levels of C1, Runx2 and OPN. (B–D) Semi-quantitative analysis of immunoblotting results of C1, Runx2 and OPN. (E) Immunofluorescence staining of OPN. (F) Immunofluorescence staining of OCN. (G) The relative protein expression levels of C1, Runx2 and OPN. (H–J) Semi-quantitative analysis of immunoblotting results of C1, Runx2 and OPN. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: Membranes were blocked with 5 % non-fat milk (BD DifcoTM, #232100) in TBST (Beyotime, #ST673) for 1 h at 25 °C and incubated overnight at 4 °C with the following primary antibodies: Osteopontin (OPN) : • Rabbit monoclonal (Proteintech, 22952-1-AP), 1:1000 • Collagen I (COL1A1): Rabbit polyclonal (Proteintech, 14695-1-AP), 1:1000 • RUNX2: Rabbit monoclonal (Proteintech, 20700-1-AP), 1:1000 • NRF2: Rabbit monoclonal (Proteintech, 16396-1-AP), 1:2000 • NQO1: Mouse monoclonal (Proteintech, 67240-1-Ig), 1:2000 • Beta Actin: Mouse monoclonal (Proteintech, 66009-1-Ig), 1:20,000 After three 10-min TBST washes, membranes were incubated with HRP-conjugated goat anti-rabbit/mouse IgG (Beyotime, #A0208/#A0216, 1:5000) for 2 h at 25 °C.

Techniques: In Vitro, Expressing, Western Blot, Immunofluorescence, Staining